Human Claudin-6 full length protein -MNP

• Catalog Number: orb1291770
• Category: Proteins
• Description: Human Claudin-6 full length protein membrane nanoparticles (MNPs)
• Target: CLDN6
• Tag: C-Flag Tag
• Reactivity: Human
• Form/Appearance: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• UniProt ID: P56747
• MW: The human full length Claudin-6 Protein has a MW of 23 kDa
• Expression System: HEK293
• Source: Mammalian
• Storage: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
• Alternative names: CLDN6
• Research Area: Epigenetics, Infectious Diseases
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

Elisa plates were pre-coated with 0.5 μg/per well purified human CLDN6 full length membrane nanoparticles. Serial diluted anti-CLDN6 monoclonal antibody (orb1291233) solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-CLDN6 monoclonal antibody binding with CLDN6 full length membrane nanoparticles is 34.36 ng/ml.

FACS analysis of CLDN6 MNPs A. Negative Control 1: CLDN6 full length membrane nanoparticles samples were stained only with Goat anti-human lgG 488 secondary antibody. B. Negative Control 2: Control membrane nanoparticles samples were stained with anti-CLDN6 antibody (orb1291233) at 2 μg/mL, followed by Goat anti-human IgG 488 secondary antibody. C. Negative Control 3: CLDN6 full length membrane nanoparticles samples were stained with anti-GPRC5D antibody (an irrelevant antibody) at 2 μg/mL, followed by Goat anti-human IgG 488 secondary antibody. D. CLDN6 full length membrane nanoparticles samples were stained with anti-CLDN6 antibody (orb1291233) at 2 μg/mL, followed by Goat anti-human IgG 488 secondary antibody.