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Human Claudin-6 full length protein -MNP
Description
Research Area
Images & Validation
−Key Properties
−| Source | Mammalian |
|---|---|
| Expression System | HEK293 |
| Target | CLDN6 |
| Reactivity | Human |
| Tag | C-Flag Tag |
| Molecular Weight | The human full length Claudin-6 Protein has a MW of 23 kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
|---|---|
| Form/Appearance | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Disclaimer | For research use only |
Alternative Names
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Elisa plates were pre-coated with 0.5 μg/per well purified human CLDN6 full length membrane nanoparticles. Serial diluted anti-CLDN6 monoclonal antibody (orb1291233) solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-CLDN6 monoclonal antibody binding with CLDN6 full length membrane nanoparticles is 34.36 ng/ml.

FACS analysis of CLDN6 MNPs A. Negative Control 1: CLDN6 full length membrane nanoparticles samples were stained only with Goat anti-human lgG 488 secondary antibody. B. Negative Control 2: Control membrane nanoparticles samples were stained with anti-CLDN6 antibody (orb1291233) at 2 μg/mL, followed by Goat anti-human IgG 488 secondary antibody. C. Negative Control 3: CLDN6 full length membrane nanoparticles samples were stained with anti-GPRC5D antibody (an irrelevant antibody) at 2 μg/mL, followed by Goat anti-human IgG 488 secondary antibody. D. CLDN6 full length membrane nanoparticles samples were stained with anti-CLDN6 antibody (orb1291233) at 2 μg/mL, followed by Goat anti-human IgG 488 secondary antibody.
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Human Claudin-6 full length protein -MNP (orb1291770)
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