Human Clara Cell Protein 16 (CC16) ELISA Kit

• Catalog Number: orb2668007
• Category: Assays and Kits
• Description: Human Clara Cell Protein 16 (CC16) ELISA Kit
• Range: 3.13-200 ng/mL
• Assay Time: 3.5h
• Sensitivity: 1.12 ng/mL
• Alternative names: SCGB1A1; CC10; CCSP; UGB; Uteroglobin; Urinary Pro
• Note: For research use only
• Application notes: standard: 200 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CC16. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CC16. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CC16, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CC16 in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: serum, plasma, tissue homogenates, cell lysates, lung lavage fluid , cell culture supernates and other biological fluids
• Expiration Date: Please enquire.