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| Catalog Number | orb775670 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chromogranin A(CHGA). |
| Reactivity | Human |
| Concentration | 2000 pg/mL |
| UniProt ID | P10645 |
| Tested applications | ELISA |
| Application notes | standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CHGA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CHGA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CHGA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CHGA in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Sandwich |
| Assay Time | 3.5h |
| Range | 31.25-2000 pg/mL |
| Sensitivity | 12.1 pg/mL |
| Sample Types | Serum, plasma, tissue homogenates and other biological fluids |
| Alternative names | CGA ELISA Kit, SP-I ELISA Kit, Parathyroid Secreto Read more... |
| Note | For research use only |
- Item 1 of 5
Anti-Chromogranin A/CHGA Antibody [orb1676428]
ELISA, FC, IHC, WB
Human
Rabbit
Polyclonal
Unconjugated
10 μg, 100 μg 
Human CHGA ELISA Kit [orb407082]
Human
62.5 ng/mL-4000 ng/mL
15.6 ng/mL
24 T, 48 T, 10 x 96 T, 96 T, 5 x 96 T- Item 1 of 1
