Human Chemokine C-X-C-Motif Receptor 4 (CXCR4) ELISA Kit

SKU: orb777384

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine C-X-C-Motif Receptor 4(CXCR4).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CXCR4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CXCR4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CXCR4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CXCR4 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.058 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CD184 ELISA Kit, Fusin ELISA Kit, NPYR ELISA Kit, HSY3RR ELISA Kit, LAP3 ELISA Kit, LCR1 ELISA Kit, LESTR ELISA Kit, NPY3R ELISA Kit, NPYRL ELISA Kit, WHIM ELISA Kit, Leukocyte-derived seven transmembrane domain receptor ELISA Kit, Lipopolysaccharide-associated protein 3 ELISA Kit

Quick Database Links

UniProt

P61073