Human Chemokine C-C-Motif Receptor 2 (CCR2) ELISA Kit

SKU: orb776794

Description

The Human Chemokine C-C-Motif Receptor 2 (CCR2) ELISA Kit uses a sandwich enzyme immunoassay to quantify Chemokine C-C-Motif Receptor 2 (CCR2) in human serum, plasma, tissue homogenates, cell lysates and other biological fluids. The kit has a detection range of 0.16-10 ng/mL and a sensitivity of 0.059 ng/mL.

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CCR2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CCR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CCR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CCR2 in the samples is then determined by comparing the OD of the samples to the standard curve

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Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.059 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

CD192 ELISA Kit, CC-CKR2 ELISA Kit, CCR2A ELISA Kit, C-C CKR-2 ELISA Kit, CCR2B ELISA Kit, CKR2-A ELISA Kit, CKR2-B ELISA Kit, CMKBR2 ELISA Kit, MCP1-R ELISA Kit, Monocyte chemoattractant protein 1 receptor ELISA Kit

Quick Database Links

UniProt

P41597