Human Chemokine C-C-Motif Receptor 7 (CCR7) ELISA Kit

SKU: orb775999

Description

The Human Chemokine C-C-Motif Receptor 7 (CCR7) ELISA Kit uses a sandwich enzyme immunoassay to quantify Chemokine C-C-Motif Receptor 7 (CCR7) in human tissue homogenates and other biological fluids. The kit has a detection range of 0.16-10 ng/mL and a sensitivity of 0.06 ng/mL.

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CCR7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CCR7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CCR7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CCR7 in the samples is then determined by comparing the OD of the samples to the standard curve

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Key Properties

• Reactivity: Human
• Sample Types: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.06 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

CD197 ELISA Kit, CCR7 ELISA Kit, BLR2 ELISA Kit, CDw197 ELISA Kit, CMKBR7 ELISA Kit, EBI1 ELISA Kit, Epstein-Barr Virus Induced Protein 1 ELISA Kit, EBV-induced G-protein coupled receptor 1 ELISA Kit, MIP-3 beta receptor ELISA Kit

Quick Database Links

UniProt

P32248