Human Ceramide Synthase 1 (CERS1) ELISA Kit

SKU: orb1146850

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ceramide synthase 1(CERS1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Ceramide synthase 1(CERS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ceramide synthase 1(CERS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ceramide synthase 1(CERS1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Images & Validation

• Application Notes:
  standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CERS1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CERS1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CERS1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CERS1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 15.63-1000 pg/mL
• Sensitivity: 6 pg/mL
• Concentration: 1000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

Ceramide synthase 1, CerS1, CERS1_HUMAN, LAG1, LAG1 homolog ceramide synthase 1, LAG1 longevity assurance homolog 1, LASS 1, LASS1, Longevity assurance (LAG1 S. cerevisiae) homolog 1, Longevity assurance gene 1, Longevity assurance gene 1 protein homolog 1, MGC90349, Protein LAG1, Protein UOG 1, Protein UOG-1, UOG 1 protein, UOG1, Upstream of GDF1,

Quick Database Links

UniProt

P27544