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Catalog Number | orb2308919 |
---|---|
Category | Proteins |
Description | Human CDCP1 (30-368) Protein, His Tag (orb2308919) is expressed from human 293 cells (HEK293). It contains AA Phe 30 - Arg 368 (Accession # Q9H5V8-1). |
Reactivity | Human |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 39.8 kDa |
Target | CDCP1 |
Expression System | HEK293 |
Expression Region | Phe 30 - Arg 368 |
Endotoxins | 1.0 EU per μg |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Buffer/Preservatives | PBS, pH 7.4 |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 39.8 kDa. The protein migrates as 53-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 72.7 kDa after removal of the signal peptide. The apparent molecular mass of CDCP1-His is approximately 100-130 kDa due to glycosylation. | |
Mammalian |
98.00% | |
39.03 kDa (predicted). Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result. |
98.00% | |
74.6 kDa (predicted). Due to glycosylation, the protein migrates to 85-100 kDa based on Tris-Bis PAGE result. |
SDS-PAGE: 86.8%; SEC-HPLC: 91.6% | |
36.4 kDa (predicted) |
> 90%, determined by SDS-PAGE | |
This protein contains the human CDCP1 (Q9H5V8-3) (Met1-Glu343) was expressed with a polyhistidine tag at the C-terminus and expressed from HEK293 Cells. |