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Human CD86 protein
Description
Research Area
Images & Validation
−| Application Notes |
|---|
Key Properties
−| Source | Mammalian cell |
|---|---|
| Biological Origin | Homo sapiens (Human) |
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human CTLA-4-Fc-Avi at 5μg/ml can bind Human B7-2-His, the ED50 of Recombinant Human B7-2-His is 1.19 μg/ml. |
| Tag | C-terminal 6xHis-tagged |
| Molecular Weight | 26.69 kDa |
| Expression Region | 24-247aa |
| Protein Length | Extracellular Domain |
| Protein Sequence | APLKIQAYFNETADLPCQFANSQNQSLSELVVFWQDQENLVLNEVYLGKEKFDSVHSKYMGRTSFDSDSWTLRLHNLQIKDKGLYQCIIHHKKPTGMIRIHQMNSELSVLANFSQPEIVPISNITENVYINLTCSSIHGYPEPKKMSVLLRTKNSTIEYDGIMQKSQDNVTELYDVSISLSVSFPDVTSNMTIFCILETDKTRLLSSPFSIELEDPQPPPDHIP |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Endotoxins | Less than 1.0 EU/μg as determined by LAL method. |
Storage & Handling
−| Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
|---|---|
| Form/Appearance | Lyophilized powder |
| Buffer/Preservatives | Lyophilized from a 0.2 μm filtered 20 mM PB, 150 mM NaCl, pH 7.2 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Human B7-2 Protein, mFc-His Tag [orb689395]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 52.4 kDa after removal of the signal peptide.
Mammalian
10 μg, 100 μg, 50 μgCD86 Antibody [orb377982]
FC, IHC, IP, WB
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
30 μl, 200 μl, 100 μl, 50 μlRecombinant human CD86 protein, C-His [orb1808096]
>90% as determined by SDS-PAGE
26.3 kDa
20 μg, 100 μg, 500 μgRecombinant human CD86 protein, C-His (HEK293) [orb1808143]
>90% as determined by SDS-PAGE
25.7 kDa
100 μg, 500 μg, 20 μgRecombinant Human CD86/B7-2 Protein, C-His [orb2968560]
>90% as determined by SDS-PAGE.
28.92 kDa
1 mg, 100 μg, 50 μg

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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Protocol Information
Human CD86 protein (orb594870)
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