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Catalog Number | orb706898 |
---|---|
Category | Proteins |
Description | Recombinant Human CD69 Protein is produced by mammalian expression system and the target gene encoding Gly64-Lys199 is expressed with a 8His tag at the N-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | CD69 |
Entrez | 969 |
UniProt ID | Q07108 |
Protein Sequence | HHHHHHHHGQ YNCPGQYTFS MPSDSHVSSC SEDWVGYQRK CYFISTVKRS WTSAQNACSE HGATLAVIDS EKDMNFLKRY AGREEHWVGL KKEPGHPWKW SNGKEFNNWF NVTGSDKCVF LKNTEVSSME CEKNLYWICN KPYK |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Alternative names | CLEC2C protein, Early activation antigen CD69 prot Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
16.8 kDa | |
Human CD69, His Tag (orb257304) is expressed from human 293 cells (HEK293). It contains AA Ser 62 - Lys 199 (Accession # AAH07037). |
Unconjugated | |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 42.1 kDa after removal of the signal peptide.The apparent molecular mass of hFc-CD69 is approximately 55-70 kDa due to glycosylation. | |
Mammalian |
Greater than 85% as determined by SDS-PAGE. | |
18.0 kDa | |
Mammalian cell |
≥90% as determined by SDS-PAGE | |
This protein contains the human CD69(Ser62-Lys199) was fused with the C-terminal His Tag and expressed in Mammalian cells. |
> 95% by SDS-PAGE. | |
KMP1099, Recombinant Human CD69 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ser 62 - Lys 199 ) of human CD69 (Accession #NP_001772.1) fused with a 6×His Tag at the C-terminus. |
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