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Catalog Number | orb1826758 |
---|---|
Category | Proteins |
Description | Recombinant human CD62E Protein with C-terminal 6×His tag |
Target | CD62E |
Tag | C-6×His Tag |
Reactivity | Human |
Form/Appearance | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
Purity | The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. |
UniProt ID | P16581 |
MW | The protein has a predicted molecular mass of 59.5 kDa after removal of the signal peptide. The apparent molecular mass of CD62E-His is approximately 100-130 kDa due to glycosylation. |
Source | Mammalian |
Storage | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Alternative names | ELAM;ESEL;SELE;ELAM1;LECAM2;selectin-e |
Note | For research use only |
Human HCRTR1 Protein, hFc Tag on SDS-PAGE under reducing condition.
98.00% | |
61.6 kDa (predicted). Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result. |
98.00% | |
61.6 kDa (predicted). Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result. |
Greater than 95.0% as determined by SDS-PAGE. | |
Sf9, Baculovirus cells |
95.70% | |
60.1 kDa (predicted); 25-35 kDa (reducing condition, due to glycosylation) |
> 95% by Tris-Bis PAGE, > 95% by SEC-HPLC | |
Biotinylated Recombinant Human E-selectin Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Trp22-Pro556) of Human E-selectin fused with His Tag and Avi tag at the C-terminal. |