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Catalog Number | orb750341 |
---|---|
Category | Proteins |
Description | Human CD3 gamma, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Gln 23 - Ser 116 (Accession # P09693-1). |
Target | CD3 gamma |
Conjugation | Unconjugated |
Tag | C-Fc |
Reactivity | Human |
Form/Appearance | Powder |
Buffer/Preservatives | PBS, pH 7.4 |
Purity | 95% |
Protein Sequence | AAI13831.1 |
UniProt ID | P09693 |
MW | 37.1 kDa |
Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 37.1 kDa. The protein migrates as 45-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Biological Origin | Human |
Expression Region | Gln 23 - Ser 116 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
NCBI | AAI13831.1 |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 36.8 kDa after removal of the signal peptide. The apparent molecular mass of CD3G-hFc is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
> 95% by SDS-PAGE. | |
Recombinant Human CD3 epsilon & CD3 delta Heterodimer Protein is produced by mammalian expression system. The target protein is expressed with sequence (Asp23-Asp126 (CD3E) & Gln23-Ser116 (CD3G)) of human CD3 epsilon & CD3 gamma Heterodimer (Accession #P07766 & P09693) fused with an Fc tag at the C-terminus. |