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Catalog Number | orb624121 |
---|---|
Category | Proteins |
Description | Recombinant Human CD276 antigen protein |
Tag | C-terminal hFc-Myc-tagged |
Form/Appearance | Lyophilized powder |
Purity | Greater than 93% as determined by SDS-PAGE. |
MW | 53.4 kDa |
UniProt ID | Q5ZPR3 |
Protein Sequence | LEVQVPEDPVVALVGTDATLCCSFSPEPGFSLAQLNLIWQLTDTKQLVHSFAEGQDQGSAYANRTALFPDLLAQGNASLRLQRVRVADEGSFTCFVSIRDFGSAAVSLQVAAPYSKPSMTLEPNKDLRPGDTVTITCSSYQGYPEAEVFWQDGQGVPLTGNVTTSQMANEQGLFDVHSILRVVLGANGTYSCLVRNPVLQQDAHSSVTITPQRSPTG |
Protein Length | Partial |
Source | Mammalian cell |
Expression System | Expression Region: 29-245aa. Protein Length: Partial |
Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized CD276 at 2 μg/ml can bind Anti-CD276 rabbit monoclonal antibody, the EC50 of human CD276 protein is 1.961-2.243 ng/ml. |
Expression Region | 29-245aa |
Endotoxins | Less than 1.0 EU/ug as determined by LAL method. |
Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4 |
Alternative names | 4Ig-B7-H3 (B7 homolog 3) (B7-H3) (Costimulatory mo Read more... |
Note | For research use only |
Application notes | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
25.2 kDa | |
Human B7-H3, His Tag (orb348789) is expressed from human 293 cells (HEK293). It contains AA Leu 29 - Pro 245 (Accession # Q5ZPR3-2). |
Unconjugated | |
95% | |
49.9 kDa | |
Human B7-H3, Fc Tag (orb334941) is expressed from human 293 cells (HEK293). It contains AA Leu 29 - Pro 245 (Accession # Q5ZPR3-2). |
Unconjugated | |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 70-75 kDa after removal of the signal peptide. | |
Mammalian |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
Unconjugated | |
95% | |
73.1 kDa | |
Human B7-H3 (4Ig), Fc Tag (orb545755) is expressed from human 293 cells (HEK293). It contains AA Gly 27 - Thr 461 (Accession # Q5ZPR3-1). |
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