Human CCR8 full length protein-synthetic nanodisc

SKU: orb1743234

Description

Human CCR8 full length protein-synthetic nanodisc

Research Area

Protein Biochemistry

Key Properties

• Source: HEK293
• Expression System: HEK293
• Target: CCR8
• Reactivity: Human
• Tag: C-Flag Tag
• Molecular Weight: The human full length CCR8 Protein has a MW of 40.7 kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing
• Form/Appearance: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization
• Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CC-CKR-8;CCR-8;CDw198;CKRL1;CMKBR8;CMKBRL2;CY6;GPRCY6;TER1

Elisa plates were pre-coated with Flag Tag CCR8-Nanodisc (0.2 μg/per well). Serial diluted anti-CCR8 monoclonal antibody (orb1291212) solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-CCR8 monoclonal antibody binding with CCR8-Nanodisc is 12.07 ng/ml.

WB analysis of Human CCR8-Nanodisc with anti-CCR8 monoclonal antibody (orb1291212) at 1 μg/ml, followed by Goat Anti-Human IgG HRP at 1/5000 dilution

Human CCR8-Nanodisc can bind Anti-CCR8 antibody (orb1291212) with an affinity constant of 1.408 nM as determined in a SPR assay.

Quick Database Links

Gene Symbol

CCR8

UniProt

P51685