Human CCR8 full length protein-synthetic nanodisc

• Catalog Number: orb1743234
• Category: Proteins
• Description: Human CCR8 full length protein-synthetic nanodisc
• Target: CCR8
• Tag: C-Flag Tag
• Reactivity: Human
• Form/Appearance: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization
• UniProt ID: P51685
• MW: The human full length CCR8 Protein has a MW of 40.7 kDa
• Source: HEK293
• Storage: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing
• Alternative names: CC-CKR-8;CCR-8;CDw198;CKRL1;CMKBR8;CMKBRL2;CY6;GPR
• Research Area: Protein Biochemistry
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

Elisa plates were pre-coated with Flag Tag CCR8-Nanodisc (0.2 μg/per well). Serial diluted anti-CCR8 monoclonal antibody (orb1291212) solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-CCR8 monoclonal antibody binding with CCR8-Nanodisc is 12.07 ng/ml.

WB analysis of Human CCR8-Nanodisc with anti-CCR8 monoclonal antibody (orb1291212) at 1 μg/ml, followed by Goat Anti-Human IgG HRP at 1/5000 dilution

Human CCR8-Nanodisc can bind Anti-CCR8 antibody (orb1291212) with an affinity constant of 1.408 nM as determined in a SPR assay.