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Catalog Number | orb391372 |
---|---|
Category | Proteins |
Description | Recombinant human CCL3 protein is produced by our E. coli. It is a single non-glycosylated polypeptide chain containing 66 amino acids with a molecular mass of 7.5 kD. |
Target | CCL3 |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Protein Sequence | SLAADTPTAC CFSYTSRQIP QNFIADYFET SSQCSKPGVI FLTKRSRQVC ADPSEEWVQK YVSDLELSA |
UniProt ID | P10147 |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Source | E. coli |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Alternative names | G0S19-1; MIP1A; SCYA3; C-C motif chemokine 3; G0/G Read more... |
Note | For research use only |
Entrez | 6348 |
Expiration Date | 6 months from date of receipt. |
> 96% as determined by SDS-PAGE and HPLC. | |
7.8 kDa | |
E.Coli |
Greater than 95% as determined by reducing SDS-PAGE. | |
7.5 KDa | |
Mammalian |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 33.6 kDa after removal of the signal peptide. The apparent molecular mass of CCL3-hFc is approximately 35-55 kDa due to glycosylation. | |
Mammalian |