Human Cathepsin K (CTSK) ELISA Kit

SKU: orb775775

Description

The Human Cathepsin K (CTSK) ELISA Kit uses a sandwich enzyme immunoassay to quantify Cathepsin K (CTSK) in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. The kit has a detection range of 0.16-10 ng/mL and a sensitivity of 0.059 ng/mL.

Research Area

Infectious Disease & Virology, Musculoskeletal & Connective Tissue Research, Protein Biochemistry

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CTSK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CTSK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CTSK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CTSK in the samples is then determined by comparing the OD of the samples to the standard curve

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Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.059 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

CTS-K ELISA Kit, CTS02 ELISA Kit, CTSO ELISA Kit, CTSO1 ELISA Kit, CTSO2 ELISA Kit, PKND ELISA Kit, PYCD ELISA Kit, Pycnodysostosis ELISA Kit, Cathepsin O ELISA Kit, Cathepsin X ELISA Kit

Quick Database Links

UniProt

P43235