Human Catenin beta-1 Protein, His Tag (MALS verified)

SKU: orb1570934

Description

Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion, as component of an E-cadherin:catenin adhesion complex. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML. Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle. Involved in chondrocyte differentiation via interaction with SOX9: SOX9-binding competes with the binding sites of TCF/LEF within CTNNB1, thereby inhibiting the Wnt signaling.

Images & Validation

• Application Notes:
  This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 62.1 kDa. The protein migrates as 60-65 kDa under reducing (R) condition (SDS-PAGE).

Key Properties

• Source: Human Catenin beta-1 Protein, His Tag (orb1570934) is expressed from E. coli cells. It contains AA Asn 138 - Glu 686 (Accession # P35222-1).
• Expression System: E. coli
• Target: Catenin beta-1
• Reactivity: Human
• Tag: N-10×His
• Molecular Weight: 62.1 kDa
• Expression Region: Asn 138 - Glu 686
• Purity: 95%
• Endotoxins: 1.0 EU per μg
• Conjugation: Unconjugated

Storage & Handling

• Storage: -20°C
• Form/Appearance: Powder
• Buffer/Preservatives: PBS
• Expiration Date: 6 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

CTNNB, EVR7, MRD19, NEDSDV, armadillo

Quick Database Links

Gene Symbol

Catenin beta-1

UniProt

P35222

RefSeq:P35222