Human Caspase-1 Protein, His Tag&Tag Free

SKU: orb3158060

Description

Caspases are an evolutionary conserved family of cysteine-dependent proteases that are involved in many vital cellular processes including apoptosis, proliferation, differentiation and inflammatory response. Dysregulation of caspase-mediated apoptosis and inflammation has been linked to the pathogenesis of various diseases such as inflammatory diseases, neurological disorders, metabolic diseases, and cancer.The members of the caspase family are grouped based on their functional contribution in inflammation (caspases-1, -4, -5, and -11) and involvement in cell death (initiation: caspases-2, -8, -9, and -10; execution: caspases-3, -6, and -7) or by their consensus recognition sequences. Inflammatory caspases recognize the sequence Trp-Glu-His-Asp and are classified as group I. Whilst group II and group III recognize Asp-Glu-X-Asp and (Leu/Val)-Glu-X-Asp, respectively.

Research Area

Cell Biology

Images & Validation

• Application Notes:
  This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 21.9 kDa & 10.4 kDa. The protein migrates as 9 kDa and 23-25 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

Key Properties

• Expression System: E. coli
• Target: Caspase-1
• Reactivity: Human
• Tag: N-10×His | Native
• Expression Region: Asn 120 - Asp 297 & Ala 317 - His 404
• Conjugation: Unconjugated
• MW: 21.9 kDa & 10.4 kDa
• Purity: 90%

Storage & Handling

• Storage: -70°C
• Form/Appearance: Liquid
• Buffer/Preservatives: 20 mM Tris, 300 mM NaCl, 0.5 M Arginine, pH 8.0
• Expiration Date: 6 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Caspase-1

UniProt

P29466

RefSeq:P29466