Human Cartilage Oligomeric Matrix Protein (COMP) ELISA Kit

SKU: orb775542

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cartilage Oligomeric Matrix Protein(COMP).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 200 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human COMP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human COMP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human COMP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human COMP in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 3.13-200 ng/mL
• Sensitivity: 1.22 ng/mL
• Concentration: 200 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

MED ELISA Kit, EDM1 ELISA Kit, EPD1 ELISA Kit, PSACH ELISA Kit, THBS5 ELISA Kit, TSP-5 ELISA Kit, Pseudoachondroplasia,Epiphyseal Dysplasia 1,Multiple ELISA Kit, Thrombospondin 5 ELISA Kit

Quick Database Links

UniProt

P49747