Human Carcinoembryonic Antigen Related Cell Adhesion Molecule 8 (CEACAM8) ELISA Kit

SKU: orb777743

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carcinoembryonic Antigen Related Cell Adhesion Molecule 8(CEACAM8).

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CEACAM8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CEACAM8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CEACAM8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CEACAM8 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 15.63-1000 pg/mL
• Sensitivity: 6 pg/mL
• Concentration: 1000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CD67 ELISA Kit, CD66-B ELISA Kit, CD66b ELISA Kit, CGM6 ELISA Kit, NCA95 ELISA Kit, Carcinoembryonic antigen CGM6 ELISA Kit, Non-specific cross-reacting antigen NCA-95 ELISA Kit

Quick Database Links

UniProt

P31997