Human Carbonic Anhydrase IX / CA9 (138-414) Protein [Out of stock]

• Catalog Number: orb334937
• Category: Proteins
• Description: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes. CAs form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa), a reversible reaction that occurs rather slowly in the absence of a catalyst. One of the functions of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. The active site of most carbonic anhydrases contains a zinc ion. There are at least five distinct CA families (α, β, γ, δ and ε). Carbonic anhydrase 9 (CA9 / CAIX) is also known as Membrane antigen MN (MN), Renal cell carcinoma-associated antigen G250, which belongs to the alpha-carbonic anhydrase family. CA9 / CAIX with an optimal activity at pH 6.49. Reversible hydration of carbon dioxide. CA IX participates in pH regulation. CA9 may be involved in the control of cell proliferation and transformation. CA-IX appears to be a novel specific biomarker for a cervical neoplasia.
• Reactivity: Human
• Tag: C-6×His
• Form/Appearance: Liquid
• Purity: 95%
• Conjugation: Unconjugated
• MW: 30.9 kDa
• Target: Carbonic Anhydrase IX
• Protein Sequence: NP_001207.2
• Source: Human Carbonic Anhydrase IX (138-414), His Tag (orb334937) is expressed from human 293 cells (HEK293). It contains AA Gln 138 - Asp 414 (Accession # NP_001207.2).
• Expression System: HEK293
• Expression Region: Gln 138 - Asp 414
• Endotoxins: 1.0 EU per μg
• NCBI: NP_001207.2
• Storage: -70℃
• Buffer/Preservatives: 50 mM Tris, 150 mM NaCl, pH7.5
• Alternative names: CAIX,CA9,CA-IX,G250,MN,P54,58N,pMW1
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 30.9 kDa. The protein migrates as 34 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human Carbonic Anhydrase IX protein