Human Carbohydrate Antigen 125 (CA125) ELISA Kit

SKU: orb776186

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carbohydrate Antigen 125(CA125).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 100 U/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CA125. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CA125. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CA125, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CA125 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 1.57-100 U/mL
• Sensitivity: 0.59 U/mL
• Concentration: 100 U/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

MUC16 ELISA Kit, Cancer Antigen 125 ELISA Kit, Mucin 16,Cell Surface Associated ELISA Kit, Ovarian Cancer-Related Tumor Marker CA125 ELISA Kit, Ovarian Carcinoma Antigen CA125 ELISA Kit

Quick Database Links

UniProt

Q8WXI7