Human cancer Antigen 27-29 (CA 27-29) ELISA Kit

• Catalog Number: orb782396
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CA 27-29(cancer antigen 27-29).
• Tested applications: ELISA
• Reactivity: Human
• Range: 1.57-100 U/mL
• Assay Time: 3.5h
• Concentration: 100 U/mL
• Sensitivity: 0.8 U/mL
• Alternative names: CA 27-29 ELISA Kit
• Note: For research use only
• Application notes: standard: 100 U/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CA 27-29. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CA 27-29. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CA 27-29, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CA 27-29 in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Expiration Date: Please enquire.