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| Catalog Number | orb777709 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calreticulin(CRT). |
| Reactivity | Human |
| Concentration | 20 ng/mL |
| UniProt ID | P27797 |
| Tested applications | ELISA |
| Application notes | standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CRT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CRT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CRT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CRT in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Time | 3.5h |
| Range | 0.32-20 ng/mL |
| Sensitivity | 0.121 ng/mL |
| Sample Types | Tissue homogenates and other biological fluids. |
| Alternative names | SLC6A8 ELISA Kit, CT1 ELISA Kit, CRTR ELISA Kit, S Read more... |
| Note | For research use only |
- Item 1 of 1
Human CALR ELISA Kit [orb406289]
Human
0.156 ng/mL-10 ng/mL
0.039 ng/mL
24 T, 48 T, 96 T, 10 x 96 T, 5 x 96 T- Item 1 of 1
