Human Calpain 1, Large Subunit (CAPN1) ELISA Kit

SKU: orb777725

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calpain 1, Large Subunit(CAPN1).

Research Area

Gastroenterology & Hepatology, Neuroscience, Protein Biochemistry

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CAPN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CAPN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CAPN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CAPN1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 1.57-100 ng/mL
• Sensitivity: 0.57 ng/mL
• Concentration: 100 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

PIG30 ELISA Kit, CANPL1 ELISA Kit, MuCANP ELISA Kit, MuCL ELISA Kit, Calpain-1 Catalytic Subunit ELISA Kit, Calcium-Dependent Protease 1 ELISA Kit, Calcium-activated neutral proteinase 1 ELISA Kit, Calpain mu-type ELISA Kit, Micromolar-calpain ELISA Kit

Quick Database Links

UniProt

P07384