Human Calcium Sensing Receptor (CASR) ELISA Kit

• Catalog Number: orb779039
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcium Sensing Receptor(CASR).
• Reactivity: Human
• Concentration: 20 ng/mL
• UniProt ID: P41180
• Tested applications: ELISA
• Application notes: standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CASR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CASR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CASR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CASR in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.121 ng/mL
• Sample Types: serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Alternative names: CAR ELISA Kit, FHH ELISA Kit, FIH ELISA Kit, GPRC2
• Research Area: Metabolism Research, Neuroscience
• Note: For research use only