Human Calcineurin (CaN) ELISA Kit

SKU: orb775746

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcineurin(CaN).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CaN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CaN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CaN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CaN in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.058 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

PPP3CA ELISA Kit, PPP3-CA ELISA Kit, CCN1 ELISA Kit, CNA1 ELISA Kit, CALN ELISA Kit, CALNA1 ELISA Kit, PP2B ELISA Kit, PP2-B ELISA Kit, Protein Phosphatase 3(formerly 2B),Catalytic Subunit,Alpha Isoformprotein Phosphatase 2B ELISA Kit, Calcineurin A Alpha ELISA Kit

Quick Database Links

UniProt

Q08209