Human Cadherin, Placental (P-Cadherin) ELISA Kit

• Catalog Number: orb1146744
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cadherin, Placental(P-Cadherin). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cadherin, Placental(P-Cadherin). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cadherin, Placental(P-Cadherin), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cadherin, Placental(P-Cadherin) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Human
• Range: 62.50-4000 pg/mL
• Assay Time: 3.5h
• Concentration: 4000 pg/mL
• UniProt ID: P22223
• Sensitivity: 37.1 pg/mL
• Alternative names: CDH3, CDHP, HJMD, PCAD, Cadherin 3, type 1
• Note: For research use only
• Application notes: standard: 4000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human P-Cadherin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human P-Cadherin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human P-Cadherin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human P-Cadherin in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Expiration Date: Please enquire.