Human C1q Tumor Necrosis Factor Related Protein 7 (C1QTNF7) ELISA Kit

SKU: orb1736739

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C1QTNF7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C1QTNF7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C1QTNF7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C1QTNF7 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area

Cancer Biology

Images & Validation

• Application Notes:
  standard: 160 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C1QTNF7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C1QTNF7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C1QTNF7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C1QTNF7 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 2.5-160 ng/mL
• Sensitivity: 1.4 ng/mL
• Concentration: 160 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CTRP7

Quick Database Links

UniProt

Q9BXJ2