Human BTLA protein

• Catalog Number: orb624122
• Category: Proteins
• Description: Recombinant Human B- and T-lymphocyte attenuator protein
• Tag: C-terminal hFc-Myc-tagged
• Form/Appearance: Lyophilized powder
• Purity: Greater than 94% as determined by SDS-PAGE.
• MW: 43.9 kDa
• UniProt ID: Q7Z6A9
• Protein Sequence: KESCDVQLYIKRQSEHSILAGDPFELECPVKYCANRPHVTWCKLNGTTCVKLEDRQTSWKEEKNISFFILHFEPVLPNDNGSYRCSANFQSNLIESHSTTLYVTDVKSASERPSKDEMAS
• Protein Length: Partial
• Source: Mammalian cell
• Biological Origin: Homo sapiens (Human)
• Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized BTLA at 5 μg/ml can bind biotinylated human TNFRSF14, the EC50 is 137.8-233.4 ng/ml.
• Expression Region: 31-150aa
• Endotoxins: Less than 1.0 EU/ug as determined by LAL method.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
• Buffer/Preservatives: Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
• Alternative names: (B- and T-lymphocyte-associated protein)(CD272)
• Note: For research use only
• Application notes: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Expiration Date: 6 months from date of receipt.

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Measured by its binding ability in a functional ELISA. Immobilized BTLA at 5 μg/ml can bind biotinylated human TNFRSF14, the EC50 is 137.8-233.4 ng/ml.

Measured by its binding ability in a functional ELISA. Immobilized Human BTLA at 2μg/ml can bind Anti-BTLA recombinant antibody, the EC50 is 1.763-3.542 ng/mL.