Human Bradykinin (BK) ELISA Kit

• Catalog Number: orb2667691
• Category: Assays and Kits
• Description: Human Bradykinin (BK) ELISA Kit
• Application notes: standard: 20 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human BK protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BK in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.12 ng/mL
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Alternative names: BK
• Research Area: Cardiovascular Research, Metabolism Research
• Note: For research use only