Human bNGF, HEK Protein

• Catalog Number: orb752930
• Category: Proteins
• Description: BNGF Human Recombinant produced in HEK293 is a noncovalently disulfide linked homodimer, glycosylated, polypeptide chain (Ser122-Arg239) containing 2 identical 118 amino acids and having a molecular mass of 26.5 kDa.
• Form/Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Buffer/Preservatives: The b-NGF was lyophilized from 1mg/ml in 20mM PB and 0.25M NaCl pH-7.5.
• Purity: Greater than 97% as obsereved by SDS-PAGE.
• Application notes: Cytokines And Growth Factors
• Source: HEK293 cells
• Biological Activity: The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line), the ED50 is < 0.04-0.4ng/ml.
• Solubility (25°C): It is recommended to reconstitute the lyophilized b-NGF in sterile distilled pyrogen free water at a concentration of 0.25mg/ml.
• Storage: Stability: Lyophilized b-NGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution b-NGF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles
• Alternative names: Beta Polypeptide, NGF, NGFB, HSAN5, Beta-NGF, MGC1
• Note: For research use only