Human BH3 Interacting Domain Death Agonist (Bid) ELISA Kit

SKU: orb775567

Description

The Human BH3 Interacting Domain Death Agonist (Bid) ELISA Kit uses a sandwich enzyme immunoassay to quantify BH3 Interacting Domain Death Agonist (Bid) in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. The kit has a detection range of 1.57-100 ng/mL and a sensitivity of 0.56 ng/mL.

Research Area

Pharmacology & Drug Discovery; Cell Biology

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Bid. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Bid. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Bid, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Bid in the samples is then determined by comparing the OD of the samples to the standard curve

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Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 1.57-100 ng/mL
• Sensitivity: 0.56 ng/mL
• Concentration: 100 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

p22 BID ELISA Kit

Quick Database Links

UniProt

P55957