Human Beta-Endorphin (β-EP) ELISA Kit

• Catalog Number: orb1807097
• Category: Assays and Kits
• Description: Human Beta-Endorphin (β-EP) ELISA Kit
• Target: bEP
• Reactivity: Human
• UniProt ID: P01189
• Application notes: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human β-EP. During the reaction, Human β-EP in the sample or standard competes with a fixed amount of Human β-EP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human β-EP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human β-EP in tested samples can be calculated by comparing the OD of the samples to the standard curve.
• Assay Type: Competitive
• Assay Time: 1.5H
• Standard: 1000pg/mL
• Range: 15.63-1000pg/mL
• Sensitivity: 5.47 pg/mL
• Sample Types: serum, plasma, Tissue homogenate and Other biological samples
• Alternative names: b-EP
• Research Area: Metabolism Research
• Note: For research use only
• Expiration Date: Please enquire.