Human Beta-2-Microglobulin (BMG/β2-MG) ELISA Kit

• Catalog Number: orb1807118
• Category: Assays and Kits
• Description: Human Beta-2-Microglobulin (BMG/β2-MG) ELISA Kit
• Reactivity: Human
• Range: 31.25-2000ng/mL
• Assay Time: 1.5H
• Target: BMG/b2-MG
• Sensitivity: 18.75ng/mL
• Alternative names: B2MG;B2M
• Note: For research use only
• Application notes: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human BMG/β2-MG. During the reaction, Human BMG/β2-MG in the sample or standard competes with a fixed amount of Human BMG/β2-MG on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human BMG/β2-MG. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human BMG/β2-MG in tested samples can be calculated by comparing the OD of the samples to the standard curve.
• Sample Types: Serum, Plasma, Tissue homogenate and Other biological samples
• Expiration Date: Please enquire.