Human Bcl2 Related Protein A1 (BCL2A1) ELISA Kit

SKU: orb781367

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bcl2 Related Protein A1(BCL2A1).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BCL2A1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BCL2A1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BCL2A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BCL2A1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.063 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

ACC-1 ELISA Kit, ACC-2 ELISA Kit, BCL2L5 ELISA Kit, BFL1 ELISA Kit, GRS ELISA Kit, HBPA1 ELISA Kit, Bcl-2-like protein 5 ELISA Kit, Hemopoietic-specific early response protein ELISA Kit

Quick Database Links

UniProt

Q16548