Human BAFF/CD257(B-Cell Activating Factor) Microsample ELISA Kit

• Catalog Number: orb2998893
• Category: Assays and Kits
• Description: Human BAFF/CD257(B-Cell Activating Factor) Microsample ELISA Kit
• UniProt ID: Q9Y275
• Application notes: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BAFF/CD257. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BAFF/CD257. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BAFF/CD257, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BAFF/CD257 in the samples is then determined by comparing the OD of the samples to the standard curve.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 78.13-5000 pg/mL
• Sensitivity: 29 pg/mL
• Sample Types: serum, plasma, tissue homogenates and other biological fluids
• Alternative names: CD257; TNFSF13B; BLYS; TALL1; THANK; TNFSF20; ZTNF
• Note: For research use only
• Expiration Date: Please enquire.