Human B-Lymphocyte Activation Antigen B7-1 (LAB7-1) ELISA Kit

• Catalog Number: orb779184
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Lymphocyte Activation Antigen B7-1(LAB7-1).
• Reactivity: Human
• Concentration: 5000 pg/mL
• Tested applications: ELISA
• Application notes: standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LAB7-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LAB7-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LAB7-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LAB7-1 in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 78.13-5000 pg/mL
• Sensitivity: 32 pg/mL
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids
• Alternative names: CD80 ELISA Kit, CD28LG ELISA Kit, CD28LG1 ELISA Ki
• Research Area: Cancer Biology, Immunology & Inflammation, Infecti
• Note: For research use only
• Expiration Date: Please enquire.