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Human AXL Receptor Tyrosine Kinase (AXL) ELISA Kit

Catalog Number: orb781402

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb781402
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to AXL Receptor Tyrosine Kinase(AXL).
ReactivityHuman
Concentration20 ng/mL
UniProt IDP30530
Tested applicationsELISA
Application notesstandard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AXL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AXL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human AXL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AXL in the samples is then determined by comparing the OD of the samples to the standard curve
Assay TypeSandwich
Assay Time3.5h
Range0.32-20 ng/mL
Sensitivity0.117 ng/mL
Sample TypesSerum, plasma, tissue homogenates and other biological fluids
Alternative namesUFO ELISA Kit, JTK11 ELISA Kit, Tyrosine-protein k
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NoteFor research use only
Human AXL Receptor Tyrosine Kinase (AXL) ELISA Kit

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