Human ATP Binding Cassette Transporter G2 (ABCG2) ELISA Kit

SKU: orb775818

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATP Binding Cassette Transporter G2(ABCG2).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ABCG2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ABCG2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ABCG2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ABCG2 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.054 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CDw338 ELISA Kit, CD338 ELISA Kit, ABC-G2 ELISA Kit, MRX ELISA Kit, BCRP1 ELISA Kit, ABC15 ELISA Kit, ABCP ELISA Kit, BCRP ELISA Kit, BMDP ELISA Kit, MXR ELISA Kit, MXR1 ELISA Kit, Mitoxantrone resistance-associated protein ELISA Kit, Breast Cancer Resistance Protein 1 ELISA Kit, Urate exporter ELISA Kit

Quick Database Links

UniProt

Q9UNQ0