Human Aspartate Aminotransferase 2 (AST2) ELISA Kit

SKU: orb867023

Description

Sandwich Human AST2 ELISA Kit

Research Area

Infectious Disease & Virology

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AST2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AST2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human AST2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AST2 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 1.57-100 ng/mL
• Sensitivity: 0.6 ng/mL
• Concentration: 100 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

GOT2 ELISA Kit, MitAAT ELISA Kit, KATIV ELISA Kit, KAT4 ELISA Kit, FABP-1 ELISA Kit, Plasma membrane-associated fatty acid-binding protein ELISA Kit, Glutamic-Oxaloacetic Transaminase 2,Mitochondrial ELISA Kit, Kynurenine Aminotransferase IV ELISA Kit

Quick Database Links

UniProt

Q8NHS2