You have no items in your shopping cart.
Cart summary
| Catalog Number | orb777791 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Asparagine Synthetase(ASNS). |
| Reactivity | Human |
| Concentration | 50 ng/mL |
| UniProt ID | P08243 |
| Tested applications | ELISA |
| Application notes | standard: 50 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ASNS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ASNS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ASNS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ASNS in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Sandwich |
| Assay Time | 3.5h |
| Range | 0.79-50 ng/mL |
| Sensitivity | 0.27 ng/mL |
| Sample Types | serum, plasma, tissue homogenates and other biological fluids |
| Alternative names | Aspartate-Ammonia Ligase ELISA Kit, Asparagine syn Read more... |
| Note | For research use only |
