Human Asc Type Amino Acid Transporter 1 (ASC1) ELISA Kit

• Catalog Number: orb778479
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Asc Type Amino Acid Transporter 1(ASC1).
• Reactivity: Human
• Concentration: 10 ng/mL
• UniProt ID: Q15650
• Tested applications: ELISA
• Application notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ASC1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ASC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ASC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ASC1 in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.057 ng/mL
• Sample Types: Tissue homogenates, cell lysates and other biological fluids
• Alternative names: SLC7A10 ELISA Kit, Solute Carrier Family 7 Member
• Research Area: Signal Transduction
• Note: For research use only
• Expiration Date: Please enquire.