Human Arachidonate-12-Lipoxygenase (ALOX12) ELISA Kit

SKU: orb778617

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arachidonate-12-Lipoxygenase(ALOX12).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ALOX12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ALOX12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ALOX12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ALOX12 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.108 ng/mL
• Concentration: 20 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

LOX12 ELISA Kit, 12-LOX ELISA Kit, ALOX-12 ELISA Kit, 12-LO ELISA Kit, 12LPG ELISA Kit, LOG12 ELISA Kit, Lipoxygenase 12 ELISA Kit, 12S-lipoxygenase ELISA Kit, Lipoxin synthase 12-LO ELISA Kit, Platelet-type lipoxygenase 12 ELISA Kit

Quick Database Links

UniProt

P18054