Human Apolipoprotein O (APOO) ELISA Kit

• Catalog Number: orb777784
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein O(APOO).
• Reactivity: Human
• Concentration: 10 ng/mL
• UniProt ID: Q9BUR5
• Tested applications: ELISA
• Application notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human APOO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human APOO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human APOO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human APOO in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.052 ng/mL
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids.
• Alternative names: Apo-O ELISA Kit, FAM121B ELISA Kit, My025 ELISA Ki
• Note: For research use only