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| Catalog Number | orb2667926 |
|---|---|
| Category | Assays and Kits |
| Description | Human Apelin 13 (AP13) ELISA Kit |
| Application notes | standard: 50 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human AP13 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AP13. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AP13 in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Competitive |
| Assay Time | 2.5h |
| Range | 0.78-50 ng/mL |
| Sensitivity | 0.21 ng/mL |
| Sample Types | Serum, plasma and other biological fluids |
| Alternative names | APLN13 |
| Note | For research use only |
Human AP13 ELISA Kit [orb405631]
Human
6.25 pg/mL-400 pg/mL
1.56 pg/mL
24 T, 48 T, 10 x 96 T, 5 x 96 T, 96 T- Item 1 of 1
- Item 1 of 1
