Human Apelin 13 (AP13) ELISA Kit

• Catalog Number: orb2667926
• Category: Assays and Kits
• Description: Human Apelin 13 (AP13) ELISA Kit
• Application notes: standard: 50 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human AP13 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AP13. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AP13 in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 0.78-50 ng/mL
• Sensitivity: 0.21 ng/mL
• Sample Types: Serum, plasma and other biological fluids
• Alternative names: APLN13
• Research Area: Cardiovascular Research, Gastroenterology & Hepato
• Note: For research use only
• Expiration Date: Please enquire.