Human AP-1 Complex Subunit Mu-1 (AP1M1) ELISA Kit

• Catalog Number: orb1994881
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human AP1M1 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AP1M1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AP1M1 in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Human
• Range: 0.32-20 ng/mL
• Assay Time: 2.5h
• Concentration: 20 ng/mL
• UniProt ID: Q9BXS5
• Sensitivity: 0.11 ng/mL
• Alternative names: AP47; CLAPM2; CLTNM; MU-1A; mu1A; Adaptor Related
• Note: For research use only
• Application notes: standard: 20 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human AP1M1 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AP1M1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AP1M1 in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: serum, plasma and other biological fluids
• Expiration Date: Please enquire.