Human Anti-Thyroid Stimulating Hormone Receptor Antiboby (TSHRAb) ELISA Kit

• Catalog Number: orb1146792
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TSHRAb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TSHRAb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TSHRAb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TSHRAb in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Human
• Concentration: 100 mIU/mL
• Application notes: standard: 100 mIU/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TSHRAb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TSHRAb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TSHRAb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TSHRAb in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 1.57-100 mIU/mL
• Sensitivity: 0.82 mIU/mL
• Sample Types: Serum, plasma and other biological fluids
• Alternative names: TSHRAb
• Research Area: Neuroscience, Signal Transduction
• Note: For research use only