Human Anti-Mannose Binding Lectin Antibody (Anti-MBL) ELISA Kit

• Catalog Number: orb781894
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with Mannose Binding Lectin(MBL) protein.
• Reactivity: Human
• Concentration: 400 ng/mL
• Tested applications: ELISA
• Application notes: standard: 400 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-MBL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-MBL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-MBL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-MBL in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Time: 3.5h
• Range: 6.25-400 ng/mL
• Sensitivity: 2.54 ng/mL
• Sample Types: Serum, plasma and other biological fluids
• Alternative names: MBL2 ELISA Kit, COLEC1 ELISA Kit, HSMBPC ELISA Kit
• Note: For research use only