Human Anterior Gradient Protein 2 (AGR2) ELISA Kit

SKU: orb776916

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Anterior Gradient Protein 2(AGR2).

Research Area

Cancer Biology, Musculoskeletal & Connective Tissue Research, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AGR2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AGR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human AGR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AGR2 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 1.57-100 ng/mL
• Sensitivity: 0.62 ng/mL
• Concentration: 100 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

GOB4 ELISA Kit, HAG2 ELISA Kit, XAG2 ELISA Kit, AG2 ELISA Kit, PDIA17 ELISA Kit, HPC8 ELISA Kit, Protein Disulfide Isomerase Family A,Member 17 ELISA Kit, Secreted cement gland protein XAG-2 homolog ELISA Kit

Quick Database Links

UniProt

O95994